|
 |
|
| The PA-4000 electroporation system opens the horizons of next generation electropermeabilization technology today. Built on a computer controlled and option expandable framework, unlocking the most difficult to transfect eukaryotic cells for ex-vivo, in-vivo, in-vitro, and other new applications has become readily accessible to researchers worldwide. Carefully designed by top experts in the electroporation field, high-quality results are consistently achievable as a result of our rigorous structural requirements.
This system provides the greatest control and process feedback available, while retaining the ability to modularize later into further applications such as cell fusion or high throughput electroporation. In addition, the intuitive Pulse Agile Interface software quickly guides even first-time users to direct success. With the PA-4000 system in your lab, there's no reason to look back.
|
 CG-4 Oligodendrocyte:
Courtesty of UCLA, MRRC, Dr. DeVellis |
|
|
Click here for Larger Image |
- Precision rectangular (square) wave electroporation
- Computer controlled operation
- Patented PulseAgile® protocol creation
- Unlimited protocol storage
- Log file automatically saved after every run
- Ionic solutions down to 10 ohms (PBS, etc.)
- Automatic safety shutdown on arc detection
- Built-in voltage and current monitors for oscilloscope verification
- Upgradeable later to Cyto Pulse option system (96-Well, etc.)
|
|
|
Rectangular (square) Wave
Rectangular wave electroporators have become industry standard because of their ability to increase transfection efficiency while maintaining viability over older and less reliable exponential decay models. Users are given the full ability to adjust parameters for voltage (electric field), pulse width, pulse number, and interval between pulses in an easy to navigate menu system.
PulseAgile Protocol
The Cyto Pulse Sciences patented PulseAgile protocol series takes the concept of square wave electroporation one step further: users are given the choice to create multiple protocols with different parameters, called groups, that are run in direct succession of one another. This is generally used to deliver a series of high voltage and short duration pulses first permeabilizing the cell membrane, followed by a series of low voltage and long duration pulses to continue delivery of the transfectant by electrophoresis.
The ultimate goal of creating these types of protocols is to deliver similar transfection efficiencies while retaining an even higher viability than standard four-parameter waves. In particular, published in-vivo applications have repeatedly been found to substantially improve delivery in addition to some ex-vivo applications.
System Monitoring
There is no mystery to whats inside the box when all necessary information can be accessed from the outside EIncluded as standard features are one each of voltage and current monitors ready to attach with an external oscilloscope in order to view real-time pulsing conditions. Using these connections is a true safeguard to know exactly how the applied waveform reflects the programmed waveform, so you never again have to wonder if your protocol ran as intended.
Furthermore, an output load estimate is provided after every run to assist in understanding how ionic the pulsing conditions were. This can be extremely valuable when debugging unexpected results due to incorrect sample washing for ex-vivo cuvette applications or proper electrode insertion for in-vivo applications. Information relevant to each executed protocol may be saved as a log file, including actual voltage levels of all pulses. Note: Estimated load is not available with the PA-101, PA-301, or PA-96W options.
Fault Detection
All Cyto Pulse Sciences equipment is designed to withstand extreme user conditions. In the event of a fault, advanced control circuitry immediately detects the source of any problem and proceeds to halt the system to prevent permanent damage. The safety of Cyto Pulse customers is our number one priority.
- Electrode Interlock improperly attached cuvettes or electrodes will cause the protocol start function to be disabled until corrected
- External Fault extremely low output load arcs will cause high voltage to be turned off and a fault screen to be displayed
- Internal Fault damaged internal system components will cause high voltage to be turned off and fault screen to be displayed
|
Cuvette Holder
|
|
|
| The heavy-duty electroporation cuvette holder will operate with all major cuvette manufacturers, although Cyto Pulse cuvettes are recommended for best results; standard cuvette sizes are available in 1 mm, 2 mm, and 4 mm electrode gaps. The insertion mechanism is notched to prevent incorrect positioning and only upon full insertion will high voltage become enabled to pulse. A large plastic barrier surrounds the cuvette field to completely block any contact with the electrodes. |
 |
In vivo Electrode Options
Cyto Pulse also offers in vivo electrodes for use with PA-3000 and PA-4000 based systems. Both parallel row needle electrode and flat plate electrodes are available.
|
| |
| General |
|
| Pulse Waveform |
Rectangular (Square) |
| Interface |
Windows Software |
| Electrode |
Cuvette, In-vivo |
| Protocol |
|
| Pulse Amplitude HV Mode |
20 - 1100 volts @ 5 volt step |
| Pulse Amplitude LV Mode |
20 - 400 volts @ 2 volt step |
| Pulse Amplitude Accuracy |
5% |
| Max E-Field (1 mm cuvette) |
11 kV/cm |
| Max E-Field (2 mm cuvette) |
5.5 kV/cm |
| Max E-Field (4 mm cuvette) |
2.75 kV/cm |
| Pulse Duration |
0.001 - 100 ms (<300 volts)
0.001 - 40 ms (<400 volts)
0.001 - 20 ms (<500 volts)
0.001 - 1 ms (<1100 volts)
|
| Pulse Interval |
0.125 - 400 sec |
| Number of Pulses |
1 - 99 |
| Number of Pulse Groups |
1 - 40 |
| Polarity Reversal |
No |
| Safety / Monitoring |
|
| Electrode Interlock |
Yes |
| Arc Control |
Yes |
| Pulse Voltage Monitor |
BNC Output |
| Pulse Current Monitor |
BNC Output |
| Electrical |
|
| AC Line |
100 - 240 VAC 50/60Hz |
| Physical |
|
| High Voltage Outputs |
MHV, Banana Plug |
| Computer Control |
RS232 |
| Dimensions |
12.6" x 11.8" x 8.2"
(32 cm x 30 cm x 20.8 cm)
|
| Weight |
23 pounds (11 kg) |
|
Included in the System |
| PA-4000 |
PulseAgile Advanced Rectangular Wave Electroporator |
| PA4-SW |
PulseAgile Application Software |
| PA4-UMAN |
PA-4000 Users Manual |
| CE-20 |
Cuvette Holder |
| CUV-M |
Cuvette Multi-pack set of 5 each: 1 mm, 2 mm, 4 mm |
| CS-L-XX |
IEC Line Cord, Serial cable, XX=country |
|
Ordering |
| PA-4000S |
Advanced PulseAgile® System |
|
