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PA-4000/PA-301S Pulse Booster System for Bacterial Transfection

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The PA-301 Pulse Booster™ option is used in conjunction with the PA-4000 electroporator to create an efficient transfection system for bacterial and yeast applications. It is intended for researchers currently experimenting with eukaryotic cell lines to expand their application boundaries and continue on the road to success. Superior results are consistently achieved as a result of our rigorous structural requirements and testing process.

This system provides the greatest control and process feedback available, while retaining the ability to modularize further to options such as cell fusion or high throughput electroporation. In addition, the intuitive Pulse Agile Interface software quickly guides even first-time users to direct success.


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  • Precision rectangular (square) wave electroporation
  • Three-fold voltage amplification for up to 3 kV
  • Computer controlled operation
  • Patented PulseAgile® protocol creation
  • Log file automatically saved after every run
  • Ionic solutions down to 10 ohms (PBS, etc.)
  • Automatic safety shutdown on arc detection
  • Built-in voltage and current monitors for oscilloscope verification
  • Easy swap to other Cyto Pulse options (96-Well, etc.)

Rectangular (square) Wave

Rectangular wave electroporators have become industry standard because of their ability to increase transfection efficiency while maintaining viability over older and less reliable exponential decay models. Users are given the full ability to adjust parameters for voltage (electric field), pulse width, pulse number, and interval between pulses in an easy to navigate menu system.
 

System Monitoring

There is no mystery to what's inside the box when all necessary information can be accessed from the outside - Included as standard features are one each of voltage and current monitors ready to attach with an external oscilloscope in order to view real-time pulsing conditions. Using these connections is a true safeguard to know exactly how the applied waveform reflects the programmed waveform, so you never again have to wonder if your protocol ran as intended.

In addition, information relevant to each executed protocol is automatically saved as a log file, including magnified voltage levels of all pulses.
 

Fault Detection

All Cyto Pulse Sciences equipment is designed to withstand extreme user conditions. In the event of a fault, advanced control circuitry immediately detects the source of any problem and proceeds to halt the system to prevent permanent damage. The safety of Cyto Pulse customers is our number one priority.

  • Electrode Interlock – improperly attached cuvettes or electrodes will cause the protocol start function to be disabled until corrected
  • External Fault – extremely low output load arcs will cause high voltage to be turned off and a fault screen to be displayed
  • Internal Fault – damaged internal system components will cause high voltage to be turned off and fault screen to be displayed
     

Cuvette Holder


 
The heavy-duty electroporation cuvette holder will operate with all major cuvette manufacturers, although Cyto Pulse cuvettes are recommended for best results; standard cuvette sizes are available in 1 mm, 2 mm, and 4 mm electrode gaps. The insertion mechanism is notched to prevent incorrect positioning and only upon full insertion will high voltage become enabled to pulse. A large plastic barrier surrounds the cuvette field to completely block any contact with the electrodes.

 

Specifications

General  
      Pulse Waveform Rectangular (Square)
      Interface Windows Software
      Electrode Cuvette, In-vivo
Protocol  
      Pulse Amplitude HV Mode 20 - 3000 volts @ 15 volt step
      Pulse Amplitude Accuracy ±5%
      Max E-Field (1 mm cuvette) 30 kV/cm
      Max E-Field (2 mm cuvette) 15 kV/cm
      Max E-Field (4 mm cuvette) 7.5 kV/cm
      Pulse Duration 0.001 - 500 µs (<400 volts)
0.001 - 400 µs (<600 volts)
0.001 - 200 µs (<1100 volts)
0.001 - 100 µs (<3000 volts)
      Pulse Interval 0.125 - 400 sec
      Number of Pulses 1 - 99
      Number of Groups 1 - 40
      Polarity Reversal No
Safety / Monitoring  
      Electrode Interlock Yes
      Arc Control Yes
      Pulse Voltage Monitor BNC Output
      Pulse Current Monitor BNC Output
Electrical  
      AC Line 100 - 240 VAC 50/60Hz
Physical  
      High Voltage Outputs MHV
      Computer Control RS232
      Dimensions PA-4000
12.6" x 11.8" x 8.2"
(32 cm x 30 cm x 20.8 cm)

PA-301
12.6" x 11.8" x 3"
(32 cm x 30 cm x 7.5 cm)
      Weight PA-4000
23 pounds (11 kg)

PA-301
14 pounds (6.35 kg)


Included in the System

PA-4000 PulseAgile Advanced Rectangular Wave Electroporator
PA4-SW PulseAgile Application Software
PA4-UMAN PA-4000 Users Manual
CE-20 Cuvette Holder
CUV-M Cuvette Multi-pack set of 5 each: 1 mm, 2 mm, 4 mm
CS-L-XX IEC Line Cord, Serial cable, XX=country
PA-301 Pulse Booster Option
PA301-UMAN PA-301 Users Manual
CS-OPT Interface Cable Set: DB25 cable, HV cable


Ordering

PA-4000/PA301S Bacteria Transfection System


 

Cyto Pulse Sciences, Inc.
810 Cromwell Park Drive, Suite T
Glen Burnie, MD 21061
410-787-1890
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